Taking to Twitter during the show, the former Big Brother housemate posted: 'Who did she s**g to get on here #CBBASHLEY'.
Despite not being fan of Ashley, Nikki went on to admit that she was interested by the other housemates, tweeting: 'Only one under 30 in the house..
Ashley has been honest about her battle with body dysmorphia and how it led her to panic attacks, previously telling Mail Online that her experiences have led to her wanting to empower and inspire other women.
She said: 'Body confidence was something I had in a cock sure way as a teenager then I lost it because there's so much pressure on women.
She has since gone on to appear on the show multiple times and finished as a runner-up on Ultimate Big Brother in 2010, before jetting over to Canada to star on their version of the series.
In 2006, Nikki admitted that she felt ashamed of her days as a £500-an-hour escort, even admitting to a liaison with rocker Pete Doherty just before her Big Brother stint.
A new report released yesterday found FGM survivors are now living in virtually every local authority in England and Wales – though Southwark in London has the highest percentage.
The only consolation I have is that I did not have sex with any of the clients.' Meanwhile, former Made In Chelsea star Ashley, admitted she was so 'nervous' about appearing on Celebrity Big Brother, having been a super-fan of the show since her younger years, as she entered the house on Tuesday.
Sharing her nerves with host Emma Willis, Ashley, who described herself as a 'secret hopeless romantic', confessed: 'I'm nervous, I'm so scared.
The Celebrity Big Brother theme for 2018 is Year of the Woman, with the Borehamwood house welcoming eight ladies onto the house during Tuesday's live launch.
Yet former Big Brother star Nikki Grahame did not seem to be embracing the theme when she lashed out at contestant Ashley James by outrageously suggesting the DJ and model must have slept with someone to bag a spot on the show.